1. What is Gas chromatography?
Ans. Gas Chromatography (GC) is a widely used analytical technique for separating and. analyzing volatile substances in a mixture
2. What is Principle Of GC?
Gas chromatography separates and analyzes volatile compounds by passing a sample through a column with a stationary phase.
The compounds separate based on their interaction with the phase, and are detected, providing information about their identity and concentration.
3. What are components Of GC System?
1. Injector
Vaporizes the sample for entry into the column.
2. Column
Separates components, typically coated with a stationary phase.
3. Carrier Gas
Moves the sample through the column.
4. Detector
Identifies separated components and generates a signal.
5. Data System
Analyzes the detector signals and creates a chromatogram.
4. What is Gas Chromatography Process?
Gas Chromatography Process
1. Sample Injection
Sample is injected into the GC system using a syringe.
2. Vaporization
Liquid sample is vaporized into a gas.
3. Separation in the Column
The sample is carried by carrier gas through the column, separating components.
4. Detection
Components are detected as they exit the column, creating a chromatogram.
5. Analysis
Retention time and peak area are used for identification and quantification.
5. What are Types of Detectors?
1. Flame lonization Detector (FID):
Detects ions formed during combustion of organic compounds. Commonly used for detecting hydrocarbons.
2. Thermal Conductivity Detector (TCD)
Measures changes in thermal conductivity as the sample passes through a heated filament.
3. Mass Spectrometer (MS)
Provides detailed information about the molecular structure of the components.
4. Electron Capture Detector (ECD)
Highly sensitive to halogenated compounds.
6. What are Types of Columns?
Capillary Columns (Open Tubular Columns)
Narrow columns with a stationary phase coating the inside. They offer high resolution and efficiency.
Packed Columns
Larger diameter columns filled with a solid stationary phase or particles coated with the stationary phase.
