61.What wavelengths can the human eye see?


Human eye is sensitive to an approximate range of wavelength of radiations from 380 nm to 760 nm.

This portion of electromagnetic spectrum is identified as Light




62.How does spontaneous combustion occur?

It is caused by a build up of sulfur in a person s body that eventually reacts to increased body heat.

63.What is the use of glacial acetic?

There are no medical uses to pure GAA, dilute concentrations can be used to remove warts or verucas; it can also be used via iontophoresis to treat bone spurs. Industrial uses include photography and the manufacturing of aspirin.

64.How do you separate the colors of ink?

The colors of ink can be separated by chromatography.

65.What Is Standard Deviation?

Standard deviation tells about the widely spread out of the measured analytical data points. Highly precised measurements are less standard deviation

66.How To Calculate Signal To Noise Ratio By This Formula 2 h/h Where H From Where And How To Measure?

The H is the height of the main peak and the h is the lowest height of a peak in the range of X 5 the main peak (around the a min peak).

67.Explain What Is A Base Line?

Base line is nothing but the detectors response to the mobile phase.( gc,hplc Base line should be stable to start a run. Un stabilized base line is called as base line noise.

Base line noise is of many types
  • Cyclic base line
  • Synchorinise noise
  • Asynchorinise noise
  • Base line drift
  • Spikes
  • Negative peaks
  • No peaks
Base line noise is attributed due to mobile phase, sample, system problems. Leaks in the system temperature variations in lab etc.


68.What Is Deference Between The Working Standard And Reference Standard?

Reference std is like USP, EP std, in this std we get COA including results of Potency by HPLC, NMR & XRPD data.

whenever there is requirement of working. std preparation first we have get API raw material from warehouse & we check assay , water content or LOD & Related substances against reference std.

Some times we are doing analysis in duplicate & get mean value. then we decide final assay or potency value & it's validity one year.

69. How Do We Fix The Sample Concentration In Hplc Method Development. What Is The Basis?

If all impurities at spec level are meeting s/n ratio more than 60 at that particular concentration as wel as no column overload.

70. Suppose We Prepared Ph Buffer Solution In Lab.and Calibrate Against Nist Solution, And Same Shall Going To Used Up To 30 Days. Is It Possible To Stable Ph And Appearance?

PH of the buffer solution will be change, as due to presence of nitrogen containing substances and also due to microbial growth. but if stored at 4 degree centi. it may used to some extent.

71.Define Octane Number?

It is the resistance to the detonation of fuel in a spark ignition engine compared to the isooctane n heptane mixture.

72.What Is Gibbs Free Energy?

It is the available energy or the greatest amount of mechanical work done by a system at constant temperature and pressure.





73. What Is Entropy?

Entropy is a measure of disorderliness. It explains the systems closeness to equilibrium.

74. Why Are Decomposition Reactions Called The Opposite Of Combination Reactions?

In a decomposition reaction a single substance splits to form two or more than two substances, whereas, in a combination reaction two or more substances combine to form a single substance So, it is opposite to each other.

75.What Is The Role of A Catalyst?

A catalyst reduces the energy of activation for a reaction by providing an alternative pathway. In this way, it speeds up the reaction and allows it to proceed under milder conditions.

76.Why Are Olefins (alkenes) Good Monomers For Polymerization Reactions?

The electrons in the weak pi bonds can be used to form strong s bonds to other monomer units
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