1. BASELINE DRIFT OR INSTABILITY
Cause: Can be caused by air bubble's, Improper mobile phase composition Or contamination in the detector
Solution: Ensure proper degassing of solvent's, check for leaks and calibrate the detector
2. POOR RESOLUTION
Cause: Overloading the column, Inappropriate Mobile Phase Column degradation ,or incorrect flow rates
Solution: Reduce sample concentration, Optimize mobile phase composition, Check column condition and adjust flow rate's
3. PEAK TAILING
Cause: Inadequate sample preparation, column overloading, or issues with the stationary phase.
Solution: Use proper sample filtration, reduce sample concentration, or replace the column if necessary
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4. NO OR WEAK SIGNAL
Cause: Detector malfunction, poor sample injection, or incorrect wavelength setting.
Solution: Check detector settings, verify the flow rate, ensure sample injection is proper, and calibrate the detector.
5. PRESSURE FLUCTUATIONS
Cause: Blocked or partially blocked column, clogged filters, or leaks in the system.
Solution: Inspect the column for blockages, replace filters, and check for leaks in the system.
6. PEAK BROADENING
Cause: Poor column packing, low flow rates, or high injection volumes.
Solution: Ensure correct column packing, optimize flow rates, and reduce injection volume
7. CONTAMINATION OR GHOST PEAKS
Cause: Contaminated solvents, sample carryover, or improper cleaning of the system.
Solution: Use high-quality solvents, clean the system regularly, and use a rinse step after each sample.
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8. COLUMN OVERHEATING OR COOLING
Cause: Inconsistent temperature control in the column oven.
Solution: Check column temperature settings and ensure the oven is functioning correctly.
9. INCONSISTENT RETENTION TIMES
Cause: Variations in mobile phase composition, changes in column conditions, or temperature fluctuations.
Solution: Regularly che c k mobile phase consistency, maintain column conditions, and stabilize the temperature.
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