Differences between HPLC and UPLC
Liquid chromatography is a technique that is utilized to separate any sample into its individual components. In this case, the chromatographic procedure involves a liquid as the moving phase. HPLC and UPLC stand for High Performance Liquid Chromatography and Ultra Performance Liquid Chromatography respectively.
It is understandable to find these terminologies quite daunting, but at its core HPLC and UPLC are both used to separate the various components of compounds found in mixtures, using high pressures to push solvents through columns. However, the similarities between the two end there and the differences between HPLC and UPLC begin.
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Differences between HPLC and UPLC
UPLC operates at considerably high pressures of 15,000 psi (psi, in this case, stands for Pounds per square inch and is a unit of pressure), while HPLC operates at relatively lower pressures of 500-6000 psi.
The key difference between HPLC and UPLC here is that UPLC has a clear advantage of HPLC, thanks to its higher pressure. This allows for shorter run times, lower solvent consumption and greater analyte separation and detection by the detector. In case you were wondering, The use of the term 'analyte' refers to any substance whose chemical constituents are being identified and measured.
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The differences between HPLC and UPLC do not end here, as it is important to gain a deeper understanding of both terms before moving further. High Performance Liquid Chromatography system is the most widely used technique for the quantification, identification and separation of the various components of a mixture. High Performance Liquid Chromatography system is particularly used in the pharmaceutical industry for the analysis of drug products, pesticides and a number of other substances. So if you intend to head into that industry, you would be wise to familiarize yourself with these terms and understand them to the best of your ability.
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