1.Do you know what is GLP?

GLP means Good Laboratory Practice. It is a framework or pattern under which research work are planned, performed, monitored, recorded, reported and archived.

2.Do you know what is Calibration Curve?

Calibration curve is the relationship between the various concentration of analyte in a suitable solvent or matrix and the signal response of the instrument.



3.Do you know what is co-chromatography?

Co-chromatography is the procedure used to detect an unknown substance by comparing the chromatic comparison with a known substance.

4.Tell me what do you know about MSDS?

Material Safety Data Sheets are used to handle chemical use in a laboratory. They are issued with every chemical that any lab uses or stocks.

5. Do you know what is blank?

Blank term is used to refer the sample tube which does not contain the analyte.

6. Do you know why GLP is followed in the lab?

Following GLP standard, minimizes the chance of error occurs due to humans.

It supports for product registration, also assures the suitability of data to the regulatory authorities.

It helps to reduce the cost of industry and governments by avoiding duplicative testing.

It helps to re create a study from the recorded data and information.

7. Tell me what are the factors on which the gas sterilization depends on?

Gas sterilization depends on factors like:

Concentration of the gas

  1. Humidity
  2. Time of exposure
  3. Temperature
  4. Nature of the load

8.Do you know what does a quality control chemist do?

Quality Control: 

Testing samples of raw materials or products either in mid stage or close to final stage to make sure that they are of high enough quality, reaching the standards and qualifications, before the product can be mass produced.

9. What Is Titration?

Titration is a process of determining molarity of a base or an acid.

10. Explain the term Aliquot and Diluent?

Aliquot: It is a measured sub volume of the original sample

Diluent: Material with which sample is diluted

11. What is Molality?

Molality is the number of solutes that are present in 1 kg of a solvent.

12. What is Titration?

Titration is a process to determine the molarity of a base or an acid. In this process, a reaction is carried out between the known volumes of a solution with a known concentration, against the known volume of a solution with an unknown concentration.

13. Mention the formula to calculate pH of a solution?

In order to calculate the pH of a solution you have to use the formula


14.What is buffer?

A buffer is an aqueous solution which has highly stable pH.

It is a blend of a weak acid and its conjugate base or vice versa, On adding a small amount of base or acid to buffer, its pH hardly changes. While when you select an acid as a buffer solution, try to use an acid that has a pH closed to your desired pH.

This will help your buffer to achieve a nearly equivalent amount of acid and conjugate base, so that it will enable to neutralize as much as H+ and OH.


15.What is a mole?

Mole is the unit used to define the number of chemical substance present in a substance.

It is the amount of substance which consists of the same number of chemical units as there are atoms in exactly 12 gram of pure carbon 12

16. Mention the formula to calculate pH.

In order to calculate the pH of a solution you have to use the formula 

pH= log [H+] or pH = log [H3O+]

17. How will you calculate how many moles of glucose present in 320 mL of 5.0 M of glucose solution?

First step:

Convert the volume from milli litres to litres

320 X (1 litre/ 1000 mL) = 0.320 L solution

Second step:

use the formula = MV = 5 moles glucose/ litre solution X 0.320 L solution = 1.6 moles of glucose present in 320 mL of solution.

18.What is the difference between Molarity and Normality?

Both techniques are used to the amount of chemical present in the solution. However, they are almost similar but differs in:

Molarity is used to know the total amount of molecules in a 1 liter solution, It is expressed as moles of a compound per liter of solution

Normality is used to know the total number of reactive units in 1 liter of solution, It is expressed in equivalent per liter

19.What is Valency?

Valency is a property of groups or atoms, equal to the number of atoms of hydrogen that the group or atom could combine with or displace it in forming compounds.

20.What is Avogadro s law?

According to Avogadro law, at the same temperature and pressure, an equal volume of gases contains the same number or molecules.

Next: Lab Chemist Interview Questions - 2

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