SOP for Calibration of Gas Chromatograph
1.0 OBJECTIVE
To check the performance of equipment for reliable and accurate results.
2.0 SCOPE
This procedure is applicable to check the performance of Gas chromatograph, installed in the quality control department.
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3.0 RESPONSIBILITY
3.1 Doing: Technical Assistant
3.2 Checking: Executive /Manager
4.0 ACCOUNTABILITY
Head of the Department
5.0 PROCEDURE
Frequency: Once in three months
5.1 Operate the instrument as per SOP
5.2 Prepare the solutions as follows.
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5.2.1 INTERNAL STANDARD: Dilute 25 ml of Ethanol to 1000 ml with purified water.
5.2.2 TEST PREPARATION: Dilute each of 1ml, 2ml, 3ml, 4ml, 5ml methanol to 100ml with internal standard.
5.3 Follow the GC parameters as per given in Annexure I.
5.4 Inject 2.0 ml of test preparation and record the chromatographs.
5.5 Calculate the ratio of the area of Methanol v/s area of Internal standard
5.6 Calculate the Correlation Coefficient
5.6.1 Correlation Coefficient should be more than 0.9900.
5.6.2 The performance of the instrument is satisfactory if the obtained Correlation Coefficient is within the given limit, otherwise follow the S.O.P.
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6.0 ABBREVIAITON
6.1 % = Percentage
6.2 ml = Mililiter
6.3 v/s = verses
6.4 GC = Gas Chromatograph
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