SOP for Calibration Procedure of U.V.Cabine
1.0 OBJECTIVE
To calibrate the equipment for reliable and accurate results.
2.0 SCOPE
This procedure is applicable to the UV Cabinet, installed in the quality control department.
3.0 RESPONSIBILITY
3.1 Doing: Technical Assistant
3.2 Checking: Executive/ Manager
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4.0 ACCOUNTABILITY
Head of the Department
5.0 PROCEDURE
5.1 Frequency: Once in 3 months.
5.2 For 254 nm
5.3 Operate the instrument as per S.O.P.
5.4 Prepare a 0.04% w/v solution of sodium salicylate in ethanol (95%)
5.5 Apply 5µl of this solution on Silica gel G with a diameter of about 5mm and examine visually.
5.5 The distance between the lamp and the plate under examination used in a Pharmacopeial test should not exceed the distance used to carry out the above test.
5.7 The lamp should be capable of revealing without doubt a standard spot of sodium salicylate.
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5.8 For 366 nm
5.9 Frequency: Once in 3 months
5.10 Operate the instrument as per S.O.P.
5.11 Prepare a 0.2% w/v solution of sodium salicylate in ethanol (95%)
5.12 Apply 5µl of this solution on Silica gel with a diameter of about 5mm and examine visually.
5.13 The distance between the lamp and the plate under examination used in a Pharmacopeial test should not exceed the distance used to carry out the above test.
5.14 The lamp should be capable of revealing without doubt a standard spot of sodium salicylate.
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6.0 ABBREVIATION
6.1 UV Cabinet = Ultra violet Cabinet
6.2 Q.C. = Quality Control
6.3 TLC plate = Thin layer chromatographic plate.
6.4 % w/v = Percentage weight by volume.
6.5 mm = millimetre
1. Check For short wave length (254nm).
Wt. of Sodium salicylate taken :-________________ mg (About 0.04 gm) dilute to100 ml with ethanol (95%).
Spotting: Diameter of about 5mm and volume of 5μl and examine visually at 254nm
Results: The lamp should be capable of revealing, without doubt, a standard spot of sodium salicylate.
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