Principle of UV Spectrophotometer
The operation of the UV Spectrophotometer is that corresponds to the Beer-Lambert law. The larger the quantity of molecules there are that can absorb light means that said light can expand and flow to give more illumination. The measurement and function of the UV spectrophotometer work collectively and can be studied together. Below are the six main components conducive to the spectrophotometer principle.
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1. Light Source :
Hydrogen-Deuterium lamps with Tungsten filament lamps are more commonly used and the ideal light source as they expand to immerse the entire UV region. The Tungsten lamps exude red radiations to the point of 375 nm, in contrast, the degree of the Hydrogen-Deuterium is less than 375 nm.
2. Monochromator:
Monochromators consist of prisms and slits. Spectrophotometers have dual beams in itself. The light emanating from the main source is distributed by the aid of circling prisms.
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The multiple wavelengths of the illumination divided by the prisms are picked up by the slits, thus the turning of the prism causes increased and constant wavelengths to be transmitted within the slots, enabling recording. The beam chosen by the slit is single colored and multiplied twice by another prism.