Determination of Shelf Life of Solutions in Laboratory
Incorrect strength or concentration of volumetric solutions, reagents and buffer solutions can alter the results of analyzed products. Solutions prepared for chemical analysis are not stable for a longer period.
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The molarities of these solutions may change after a period of time. The shelf life of solutions depends on the nature of the compound and the solvent also. Therefore, it is necessary to validate the stability period of these solutions individually in which these have to consume. Validation of shelf life is a mandatory GLP and regulatory requirement. There are different ways to assign the shelf life of solutions; following are the simplest methods for the same.
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Volumetric Solutions:
Prepare the volumetric solution and allow standing. After 24 hours determine the molarity of the solution in triplicate calculating the mean of the results and continue the determination at an interval of 3 days for 15 days. Determine the RSD of all 5 values. If RSD of all 5 values remains less than 1.0, the shelf life of solutions should be assigned 15 days otherwise RSD shall be calculated with first 4 values and shelf life should be assigned 12 days. If the RSD of first 4 values remains more than 1.0, RSD shall be calculated with first three values and shelf life of solutions should be assigned accordingly.