SOP for Calibration of Atomic Absorption Spectrophotometer (AAS)

 SOP for Calibration of Atomic Absorption Spectrophotometer (AAS)

1.0 Objective

To check the performance of the equipment for reliable and accurate results.

2.0 Scope

This procedure is applicable to check the performance of the atomic absorption spectrophotometer installed in the quality control department.

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3.0 Responsibility

3.1 Doing: Technical Assistant

3.2 Checking: Executive /Manager

4.0 Accountability

Head of the Department

5.0 Procedure

Frequency: Once in a month

5.1 Operate the instrument as per respective standard operating procedure.

5.2 Use Cu standard for radiations.

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5.3 Prepare 0.5 mcg/ml, 1.0 mcg/ml, 1.5 mcg/ml, 2.0 mcg/ml and 2.5 mcg/ml in water.

5.4 Aspirate the above solution and measure the absorbance.

5.5 Calculate the correlation coefficient by the instrument/computer.

5.6 Correlation coefficient should not less than 0.9900 %.

5.7 The performance of the instrument is satisfactory if the obtained correlation coefficient is within the given limit otherwise follow the S.O.P.

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6.0 ABBREVIATIONS

6.1 % = Percentage

6.2 mcg= microgram

6.3 ml= millilitre

6.4 cu= copper

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