SOP for Calibration of Plunger Operated Pipette
1.0 Objective
To calibrate the plunger operated pipette for reliable and accurate results.
2.0 Scope
This procedure is applicable to all plunger operated pipettes used in Q. C. department.
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3.0 Responsibility
3.1 Doing: Technical Assistant
3.2 Checking: Executive /Manager
4.0 Accountability
Head of the Department
5.0 Procedure
Frequency: Every six month
5.1 On receipt of plunger operated pipette, perform calibration of the same.
5.2 Before testing, condition the pipette by pipetting purified water for five times and discard pipette tip.
5.3 Mount a new pipette tip and rinse with the purified water.
5.4 Weigh the beaker having a weight less than 4 gm on a microbalance.
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5.5 Withdraw three different volume (maximum, minimum and middle of the range) of purified water and pipette out in to dry beaker which is previously weighed.
5.6 Calibrate the pipette by wt/wt and convert to ml by using standard wt/ml at the temperature of water at the time of calibration referring Annexure –II.
5.7 Repeat the above exercise for three times and record the results in Annexure-I.
5.8 Acceptance Criteria
For 20 µl
50 µl = + 0.7 %
100 µl
200 µl
For 500 µl = + 0.5%
1000 µl
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6.0 ABBREVIATIONS
6.1 SOP = Standard Operating Procedure
6.2 % = Percentage
6.3 gm = gram
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