Calibration of Volumetric Glassware used in Pharmaceuticals
Calibration of volumetric flasks
Weigh a clean & dry volumetric flask. Calibrate the flask by filling it with recently boiled and cooled distilled water at room temperature up to the mark and weigh the flask. Determine the volume in ml by the formula;
Volume (in ml) = (Weight in g / 0.99602)
Where 0.99602 g is the weight of 1 ml water determined at temperature 25°C.
[ads id="ads1"]
For the volumetric flasks having capacity more than 100 ml, dispense the water from 100 ml burette (class A) in multiple steps if required and note the final volume (i.e. total volume dispensed).
Calibration of one mark pipette / graduated pipette / burette:
Calibrate the one mark pipette / graduated pipette/burette by filling it with recently boiled and cooled distilled water at room temperature up to the mark. Collect the water from the pipette into a clean, dry previously weighed beaker [For burette – drain the water up to the lower mark]. Take the weight of beaker with water. Check the weight of water by subtracting the wt. of empty beaker from the weight of beaker with water.
[ads id="ads1"]
Determine the volume in ml by the formula;
Volume (in ml) = (Weight in g / 0.99602)
Where 0.99602 g is the weight of 1 ml water determined at temperature 25°C.
Frequency: Initially when volumetric glassware is purchased.
[ads id="ads1"]
Note: When the volumetric glassware does not comply with the requirement as specified above, the volumetric glassware should be returned back to the party.